Flow Injection Spectrophotometric and Reverse Phase-High Performance Liquid Chromatography Determination of Ranitidine.Hydrochlrid inPharmacetical Preparations

Abstract

This study describes development of a new flow injection spectrophotometric method and
a reverse phase-high performance liquid chromatography RP-HPLC method for the
determination of Ranitidine.HCl (RH) in aqueous solution and in pharmaceutical
preparations.
The FIA method was based on the reaction of RH with ferric nitrate and the produced Fe(II)
was reacted with potassium hexacyanoferrate (III) forming a Prussian blue colored dye that
has a maximum absorption at 800 nm. Linearity was in the range of 2-80 μg.ml-1,
correlation coefficient of 0.9996, detection limit of 0.613 μg.ml-1 and relative standard
deviation percent of 2.17%. The method was applied successfully to the determination of
RH in pharmaceutical preparations with a recovery of 100.23 – 101.04%.
RP-HPLC method with a Supelco BDS-C18 –DS (25 cm x 46 mm i.d) analytical column
(5 μm particle size) and isocratic elution with a mobile phase containing 30% methanol in
0.05 M KH2PO4 buffer (pH 6.0), at a flow rate of 1 ml.min-1, 20 μL sample loop, and a UV
detector at λmax 330 nm. The calibration graph was linear in the range of 0.1 – 1.5 μg.ml-1
with the correlation coefficient of 0.9994. The method was applied successfully for the
determination of pharmaceutical preparations containing Ranitidine.HCl with a recovery of
100.63–101.31%.