Flow Injection Spectrophotometric Determination of Copper in the environmental and biological samples using iodine-starch complex as chromogenic agent

Abstract

A simple and facile flow injection spectrophotometric method was developed for the
determination of copper (II) in aqueous and biological samples. The method is based on the
oxidation – reduction reaction between copper (II) and iodide ion in acidic medium to form
iodine, the released iodine reacts with soluble starch solution to form iodine starch blue
complex which is detected at 600 nm wavelength .Chemical and physical parameters were
investigated. The calibration curve obeyed Beer's law between ( 1.0– 25 μg.ml-1) with a limit
of detection 0.6 μg.ml-1.The relative standard deviation was 0.62% for 10.0 μg.ml-1 copper
solution (n=8) with sampling rate of 35 samples / h