A Study Of Kinetic And Thermodynamic On Plasma Ceruloplasmin In Patients With Bladder Cancer

Abstract

The levels of plasma ceruloplasmin (CP) in patients with bladder cancer, bladder
stones as pathological control and normal subjects were determined .The study include the
kinetic and thermodynamic properties. The study include (35) patients suffer from bladder
cancer, (25) patients suffer from bladder stones disease and (25) normal subjects. The
statistical analysis showed a significant increase (P < 0.05) in the activity of plasma CP in
patients with bladder cancer as compared to pathological control and normal subjects , the
optimum conditions of enzyme activity showed a 9.26 mmole/L concentration of pphenylene
diamine (PPD) ( substrate) under incubation time of 15 min & 370C . Estimation
of some kinetic & thermodynamic parameters of plasma CP enzyme reaction, indicated that :
the km values for the plasma CP were lower in bladder cancer patients than in normal
subjects, while the Vmax values for the CP reaction found to be higher in bladder cancer
patients than in normal, the forward reaction rate constant (k1) for CP decreased in bladder
cancer patients. The disease affected (increased) the half-life time (t1/2) of CP reactions , the
Hill coefficient (n) values revealed that there was a significant difference in the case of
bladder cancer patients as compared with those of the control groups, the activation energy
Eact.* for CP reaction decreased in bladder cancer patients compared with normal group
subjects , the ΔH* values for CP enzyme in normal subjects and bladder cancer patients were
positive that confirmed the reaction to be endothermic. The ΔS* values for CP enzyme in
normal subjects and bladder cancer patients were negative ,whereas the ΔG* values for CP
enzyme-substrate reactions in normal and bladder cancer patients groups were positive.