Differential Pulse Polarographic Assay of Azathioprine in Serum and Urine

Abstract

The polarographic behaviour of imuran (azathioprine) have been studied , the drug
give a well sensitive defined double peaks at about (-0.4V, -0.96V) in carbonate buffer
solution at pH 7.4 and at (-0.54V, -1.07 V) in serum – carbonate buffer and at (-0.42V, - 1.03
V) in urine – carbonate buffer.
The lowest determined concentration was (8.7x10-7 M) in serum-carbonate media and
(15.2x10-7 M) in urine-carbonate media. The method can be successfully applied to
determination of azathioprine in vivo. The relation between concentration and diffusion
current (Ip) was linear for all solutions.