Inhibition of the mutagenic activity of cyclophosphamide using green tea extract

Abstract

The drug cyclophosphamid CP has harmful genetic effects when used with higher
doses. For this reason, this study was carried out to determine these harmful effects,
and try to reduce these effects by using the metabolic extract of green tea. The
extract was characterized using TLC .The results indicate that the extract contained
various compounds. Rats were treated with different doses of CP and DNA
extracted from WBC and analyses with DNA electrophoresis. The results showed that there was a direct relationship between the dose of CP and level of DNA lysis.
The results also showed that giving the green tea extract before the CP is the best
way in reducing the effects of CP on DNA. These results may indicate that the
extract works as antimutagenic agent, while when given with the CP , the extract
may works as desmutagenic agent. The MI results showed that treating the rats with
CP alone reduced MI (23.33), when compared with the negative control (39.33) .
When the extract was given before the CP, MI was (34.33), while the MI was
(30.66) and (25.66) when the extract was given with and after the CP, respectively.
Chromosomal studied in bone marrow cell of rats indicate that the CP at 20 mg / Kg
causes increase in the level of chromosomal aberrations. These aberrations increase
and decrease in the number of chromosomes, ring chromosome, brakes in the
chromosomes and chromatiedes and in the centromere region, and other aberrations.
However when the green tea extract was given before or with the CP the level of
chromosomal aberrations was reduced in comparison with the positive control.
From the results of present study, we conclude that the green tea extract had
antimutagenic effect when given at the dose of 1 g / Kg before and with the CP.