Spectroscopic Studies of Prostate Specific Antigen (PSA) Molecule

Abstract

Spectroscopic studies in the U.V region were carried out on human PSA.
The U.V spectra of PSA, was obtained and revealed that PSA have a characteristic
spectrum. The effect of pH and polarity on PSA U.V spectra was studied.
Spectrophotometric pH titration and solvent perturbation were carried out and the data
revealed that about 83.3% of tyrosine residues are located on the surface of the PSA
molecule, while about 16.7% of tyrosine residues are buried interior the folded
structure of the PSA. About 27.4% of histidine residues is located on the surface of
the PSA molecule, while about 72.6% of histidine residues is embedded in the interior
region of the PSA molecule.