Development of an Immunoradiometric Assay for Determination of PSA in Benign and Malignant Brest Tumors

Abstract

Total prostate specific antigen (t-PSA) levels were measured in sera and
tissues of three groups of patients with breast tumors using immunoradiometric assay
(IRMA). Group I consisted of 29 women suffering from benign breast tumors. Group
II and III consisted of 21 and 27 women suffering from pre- and postmenopausal
malignant breast tumors. In addition to that, another group of 32 women were used as
control. The results obtained revealed that the levels of serum t-PSA in group I was
increased significantly (P< 0.0001) whereas in group II and III, the levels of serum t-
PSA were slightly significant (P< 0.05). An immunoradiometric assay procedure was
developed for the determination of t-PSA in the three groups. Total PSA (t-PSA) level
in tissues of patients with group I was significantly higher than in group II and III (p<
0.001) whereas the level of t-PSA in tissues of patients with group II and III were no
significant (P< 0.1). The results obtained revealed higher incidence of t-PSA in group
I than those in group II and III.