Biological production of cellulase enzymes from fungi using the SSF method on textile waste

Abstract

Two cellulolyltic fungi (T.harzianum) isolated from the Pesticide (biocout)
And (A.nadulans) was isolated from a soil of tomato farm, were grew on PDA ( 4
days ,pH = 5.2 and 30C°),for cellulase production. Cellulase produced using SSF
technology by incubation of cotton textile residues (carbon source).
Three type of cellulase were produced :
- Endoglucanase give high activity on the 7th day of incubation for T.harzianum
(1.54 U/ml) , while A.nadulans give at the same day ( 1.48U/ml) , and they give
total activity (15.4 and 14.8/gm carbon source ) respectively.

Exoglucanase give an observed highest activity for T.harzianum in the 7th day of
incubation (0.416U/ml), while A.nadulans have (0.159U/ml) in the same day.
Having total activity (4.16 and 1.59/gm carbon source ) respectively.
- While β-glucosidase on the other hand , A.nadulans give an enzymatic activity
higher than T.harzianum ( 6.45 and 5.61 ) U/ml respectively , and the total activity
values for the two fungus
are ( 64.5 and 56.1 ) / gm carbon source.