Extraction and purification of Urease from Proteus mirabilis

Abstract

Urease was extracted from uropathogenic P.mirabilis using Lauria agar and
then purified by ion- exchange chromatography using DEAE-cellulose and gel
filtration chromatography on Sephacryl S-200 column. The purification fold was
13.86 and the yield was 45.4%.
The molecular weight of urease was estimated by gel filtration and SDSpolyacrylamide
gel electrophoresis. The native molecular weight of the enzyme using
gel filtration was about 174000 Daltons whereas SDS-polyacrylamide gel
electrophoresis revealed three bands and the molecular weight for these bands were
(53.70, 31.68, 20.89) kDa respectively.