Separation of the aucucin compound (Ancubin) from the extract of plantain (PLANTAGO MAJOR L.) using gel filtration chromatography

Abstract

The aim of this study is to devising a Novel Method (Gel Filtration) to isolate the
bioactive component Aucubin from Plantago Major plant (one of Plantoginaceae
family) which grows in Iraq.
Different extracts of leaves and stems of Plantago Major plant were prepared.
These extracts include: cold and hot water and ethanolic extracts for fresh and dried
samples of plant for each of these solvents.
The separation technique includes gel filtration using a column of Sephadex G-25
with three mobile phases (water, Tris HCl pH=7 and Tris HCl pH=8). While the
identifications are completed by molecular absorption spectrophotometer and thin
layer chromatography (TLC).