FI-Spectrophotometric Determination of Adrenaline in Pharmaceutical Formulations Via Oxidative Coupling Reaction with Tyramine and Potassium metaperiodate

Abstract

A batch and flow injection (FI) Spectrophotometric methods have been
developed for the determination of adrenaline in aqueous solution and in
pharmaceutical ampoules preparation .The methods are based on the reaction of
adrenaline with tyramine in presence of potassium metaperiodate as oxidizing agent in
neutral solution .The water soluble orange colour produced was measured at 475 nm
.Linearty was observed from 0.6 – 25 and from 1 – 70 μg.ml-1 adrenaline with
quantification limits of 0.23 and 0.47 μg.ml-1 adrenaline by batch and FI procedure
respectively . The effect of chemical and physical parameters have been carfully
considered and the proposed methods were successfully applied to the determination
of adrenaline in ampoules formulation .