Isolation, identification and antibacterial activity of flavonoid compound from vitex aguns castus L. seed

Abstract

We isolated the flavonoid compound from vitex aguns castus L. seed as red powder at (50oC).
The chemical and physical properties were studied by using thin layer chromatography (TLC),
IR-spectrum, ultraviolet-visible spectrum, melting point (m.p) and qualitative testes of it. The
Isolated flavonoid compound is shown high antibacterial activity against types of standard strains
of bacteria (Staphylococcus aureus NCTC 6571 and Escherichia coli NCTC 5933). The
minimum inhibition concentration (MIC) of the isolated flavonoid compound has against gram
positive and negative bacteria equal to (1 μgm/ml). We prepared flavonoid complex, where it
shows high degree of inhibiting the test organism comparable to the isolated flavonoid
compound alone.