Determination of Ascorbic acid Via Semi automated On –line merging zone technique

Abstract

A Flow injection analysis method for determination of ascorbic acid as
Molybdenum blue is described. Orthophosphate ion, Molybdenum and ascorbic acid were
reacted to form molybdenum blue then the absorbance was measured at 660 nm.
The typical analytical working curve obtained under the optimized experimental
conditions were rectilinear form (1 x10^-3 _1x 10^-4 mM) and the limit of detection was
11.4ng .Measurement of one sample (with out measurement time loading) takes 1minute.
The method setup offers a precise, sensitive, selective and high sample through put
technique for determination of Ascorbic acid in standards sample .It compared well with
conventional standard (1) spectrophotometer method .