Determination of Chloramphenicol from pure and pharmaceutical preparations using FIASpectrophotometric methods

Abstract

Flow injection spectrophtometric method was described for the trace
determination of chloramphenicol. This method depends upon the reduction of the nitro
group of the chloramphenicol to the amino group using Zn- reductor mini-column, then
diazotization of the amino group with nitrous acid and coupling with 8-hydroxyquinoline
in alkaline medium to give an intense water soluble and stable azo dye with a maximum
absorption obtained at a wavelength of 485 nm. The linear calibration curve was applied
in the range of (0.5- 50 μg ml-1). The relative standard deviation was estimated as ≤ 1.4%
for (30 μg ml-1) chloramphenicol solution of 10 successive injections. This method was
found to be easy, simple, reproducible, high precession and very sensitive for the
determination of chloramphenicol in pure form and in pharmaceutical preparations
comparing with British pharmacopoeia method.