Study of the Effect of Storage on the Level of Bilirubin and Some Serum Proteins

Abstract

Serum total protein (S.T.P.), aspartate aminotransferase (AST), alanine
aminotransferase (ALT), albumin, and serum total bilirubin are of biological and chemical
importance in the diagnosis and follow up in different diseases. A variety of changes in the
concentration of these substances with the time of storage were found with time. This
research studied the possible changes in the concentration of these substances with time.
The results showed a decrease in all the biochemical substances that measured in the
research and the amount of decrease depends on the time. The decrease depends on the
chemical type of each parameter. Aminotransferases enzymes showed the most significant
changes with storage are noticed in enzymes.
It was found a negative relationship between the storage time and each of the
measured biochemical parameters in serum. The changes in serum total bilirubin explained
by the possible effect of light on the chemical transformation of bilirubin into lumirubin.
Serum proteins, especially enzymes, decrease due to the possible denaturation caused by
freezing and thawing processes. It is recommended that the analysis should be done
immediately after blood aspiration. If the sample delayed then the results should corrected
according to the ratios that obtained in this work.