Determination of Chromium in Biological Materials Using Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP/AES)

Abstract

Many concentrated acids or their mixtures were used to compelet the digestion
of the certified reference material type(MA-A1) which was used for the determination
of chromium in it using Inductively Coupled Plasma-Atomic Emission Spectroscopy
(ICP/AES).
It was found that the mixture (HClO4:H2O2)(1:1) was to be the best for chromium
determination with recovery of (98.19)% and (HCl:H2O)(1:1) as a second proper acid
for chromium analysis .This method was applied on twenty tissue samples of patients
with breast tumors aged (15-60) years to determine chromium concentration .
Chromium concentrations in sera samples of the same patients were also measured
and deficiency in chromium concentration values were appeared in sera of patients
with breast cancer compared with benign cases .