ADENOSINE 3' , 5' – CYCLIC MONOPHOSPHATE : EXT RACION AND PURIFICATION FROM URINE OF β – THALASSAEMIA PATIENT
Abstract
Cyclic adenosine 3',5'-monophosphate has been extracted and purified
using column chromatography ( Dowex 50-w column of 1.5x 5 cm dimentions ). Levels
of the purified adenosine cyclic monophosphate peak was determined and it was seen to
be elevated in urine of thalassaemic patients in comparison with that of healthy controls
objects. The purified peak was identified using UV analysis.