A novel method for spectrophotometric determination of isopropamide iodide in pharmaceutical formulations Application to content uniformity testing

Abstract

A simple, accurate, economical, and rapid spectrophotometric method for the determination of isopropamide iodide in pharmaceutical preparation (tablets) has been developed. The method is based on the reaction of isopropamide iodide and sodium nitrite in sulfuric acid medium to form iodine which shows maximum absorbance at 449 nm. Beer,s law was obeyed in the concentration range ) 20-240μg/ml). The molar absorptivity and Sandell,s sensitivity of the colored complex are 0.67×103 l/mol.cm. and 0.717 ng/cm2 respectively .The limits of detection and quantization are 5.8 and 17.4 μg/ml respectively. The analytical parameters were optimized and the method was successfully applied to the determination of isopropamide iodide in pure form and its tablets form.