Synthesis of N-(4-Chlorophenyl)-2-methoxy-4-methylbenzamide and Spectrofluorometric Study

Abstract

N-(4-Chlorophenyl)-2-methoxy-4-methylbenzamide (3) is synthesized from reacting
carboxylic acid (1) with 4-chloroaniline (2) by using N,N’-dicyclohexylcarbodiimide (DCC) and
1-hydroxybezotriazole (HOBt) as coupling reagent. The product is confirmed by IR, 1H-NMR
and elemental analysis . The conditions of fluorescence behaviours are studied such as solvent,
pH, temperature, time, concentration effect and binding constant at λex340 nm and λem 380 nm.
The best fluorescence intensity is obtained at pH = 5 and temperature 25 0C, while the
fluorescence intensity remains stable with increasing of time. The LOD, LOQ, S.D and R.S.D%
of compound 3 are found 0.2691 mg.L-1, 0.898 mg.L-1, 0.591 and 1.369 respectively.