Square Wave Voltammetric Method as a Tool for Determination of Cholesterol Quantity in Human Blood Serum

Abstract

A new method for the determination of Cholesterol in human blood serum based on
Square Wave Voltammetry (SWV) has been described. This method is relay on the decrease
in the reduction peak of 4-aminoantipyrine appeared at (+0.004 V) vs. (Ag/AgC1,3M KCl) as
a reference electrode using Phosphate buffer (pH 7.0) as supporting electrolyte, the method is
sensitive and rapid. The procedure was successfully applied to the determination of
cholesterol in various blood samples represent different cases such as: Hypothyroidism,
Nephrotic syndrome, Obstructive Jaundice & Diabetes Mellitus. The results have been
compared with those obtained from the spectrophotometric method, a good agreement
between the two methods has been obtained with correlation coefficient (r = 0.9986).