Spectrophotometric Determination of Cefixime Through Schiff’s Base System Using Vanillin Reagents Inpharmaceutical Preparations

الملخص

A simple, rapid, accurate and sensitive spectrophotometric method for the
determination of cefixime has been developed. The method is based on the formation
of the schiff’s base between the primary amino group present in cefixime and
aldehyde group present in the vanillin reagent to produce a yellow colored complex
having maximum absorption at 414 nm. Beeˈs law was obeyed over the concentration
range of 2-20μg/ml,with molar absorptivity of 8.22x104 l/mol.cm. The present
method is considered to be simple because it does not need heating, hydrolysis and
solvent extraction steps. The ingredients often formulated with cefixime have been
shown not to interfere, and is suitable for the routine determination of cefixime. The
proposed method has been successfully applied for the determination of cefixime in
pure form and in pharmaceutical preparations(capsules)