Spectrophotometric determination of pyridoxine hydrochloride via complexation with Fe( III )in pharmaceutical and environmental wastewater samples

Abstract

To develop spectrophotometric method for the determination of pyridoxine
hydrochloride in commercial dosage forms and industrial wastewater samples. The
method is based on the chelation of the drug with Fe III to form red colored metal
chelate at room temperature which absorbs maximally at 465 nm. Beer s law is
obeyed over the concentration range of 2-28 μg/ml with molar absorptivity and
Sandell s sensitivity of 0.534x104 l/mol.cm and 0.035μg/cm2 respectively, relative
standard deviation (RSD)is less than2.0 (n=10). The method is applied successfully
for determination of pyridoxine hydrochloride in some pharmaceutical formulations
(tablets and injection)and industrial wastewater samples. A statistical comparison of
these results with those of official method using (t and F) values at 95% confidence
level shows good agreement and indicates no significant difference in the precision.
So that the proposed method can be used as a routine quality control for determination
of pyridoxine hydrochloride in pure form, pharmaceutical formulations and industrial
wastewater samples.