U.V. Derivative spectra studies of some Schiff’s Bases of Amino Acids complexes- Trace Amount Determintion.

Abstract

According to the biological and industrial importance of Schiff bases derived
from amino acids, the U.V. derivative spectra were studied for Schiff bases derived
from Leucine amino acid and their complexes with Ni (II) and Co (II) in ethanol as
solvent. The plot of the molar concentration versus the absorbance result in a straight
line relationships obeys the Beer’s Lambert law with in certain ranges of
concentrations. The Zero, first and second order derivative spectra were recorded and
the integrated area under the peaks estimated for a solutions of different
concentrations which employed for quantification of the complexes of these Schiff
bases and shows a relative enhancement in the determination limits as compared with
Zero- order technique.