Flow injection spectrophotometric determination of Tetracycline hydrochloride in pharmaceutical samples

الملخص

Tetracycline hydrochloride (TCH) was determined spectrophoto-metrically in
the pure form and in the pharmaceutical formulations using flow injection analysis
(FIA). The automated method is based on the reaction between TCH, sodium
nitroprusside and hydroxylamine hydrochloride in alkaline medium. The product
absorbed maximally at 529 nm. The calibration graph of TCH is linear from 25-1000
μg mL-1 with detection limit (S/N=3) of 7.589 μg mL-1 and sample through put of 69
h-1. The various chemical and physical variables were optimized and the proposed
procedure was applied successfully for estimation of drug in different commercial
forms.