High Performance Liquid Chromatographic Method for the determination of Diclofenac sodium in pharmaceutical preparations and in Environmental Samples

الملخص

Rverse phase high performance liquid chromatography (HPLC)method has been
developed for the determination of diclofenac sodium in pharmaceutical preparations and
environmental water samples.. Chromatography was carried out on supelco C18 column
(25cm×4.6m.m), 5μm. Using methanol as mobile phase. The flow rate was 1.0 ml/min
with Uv-detection at 283 nm. Separation was completed within 2.9 min. Calibration curve
was linear coefficient correlation 0.998 over a concentration range of (5-80μg/ml). The
relative standard deviation ( RSD) was found <1%.The proposed method was
successfully applied to the determination of diclofenac sodium in pharmaceutical dosage
forms and in environmental water samples.