The Effect of pretreatment and time of Storage on the determination of selenium by AAS in tissues of patients with breast tumors

المؤلفون

  • Rafa K. AL-Kubaisy
  • Mohammed A . AL.Hamdany
  • Samera H . Hamad
  • Yusra A.AL kaliq
  • Muneer.A.AL.Da'amy

الملخص

This study involves an investigation on the effect of pretreatment using many
acids or their mixtures on the determination of selenium in a standard reference
material of tissue.
The mixture (HClO4: H2O2) (1:1) gave the best accuracy in the determination
of selenium concentration in the standard sample, the optimum conditions for the
other parameters (Temperature & time of storage of the tissue in solutions (formalin
& normal saline) were also evaluated.
The standard calibration curve revealed a straight line and high sensitivity
indicated by detection limit (DL=0.3 X 10-3 ug / ml. ) and % recovery = 96.2 .
Selenium can be measured in tissue samples which kept in formalin up to 6
days for both Benign & malignant tissues . But, in normal saline up to 4 days in both
benign & malignant tissues can be tolerated .
Higher values for selenium concentration in malignant tissue compared with
benign tissue are found

التنزيلات

منشور

2023-06-17