UV-derivative Spectra of Co-enzyme Q0. Determination of trace amounts.

Abstract

UV derivative spectra were used for the direct quantitative determination of
coenzyme Q0, ubiquinone Q0, in aqueous solution, the quantification was
accomplished according to the integrated area under the peaks and peak amplitude.
The zero-order spectrum of coenzyme Q0 show an absorption band at 268 nm, with
molar extinction coefficient εmax=15162 l.mol-1cm-1, the determination limit was 0.02-
18.2 μg/ml with R2=0.9999 and relative standard deviation RSD=0.1382 %.
The first, second, and third order spectra were recorder for coenzyme Q0 in
water at different concentrations and the determination limits were estimated.