Evaluation of Ceruloplasmin Oxidase Activity and C-Reactive Protein in the Sera of Patients With Diabetes Mellitus

Abstract

The type two Diabetes Mellitus patients (independent DM2) was used to
investigate the level of C-reactive protein CRP & ceruloplasmin oxidase activity CP. The
study aimed to compare the effects of altering the glycemic index or the amount of
carbohydrate (serum glucose) with C-reactive protein and CP activity in patients compare
with control group. As a marker of systemic inflammation, raised CRP concentrations are
still within the normal range have been associated with an increased inflammation of
Diabetes Mellitus DM.
The our experimental data reveal that ceruloplasmin oxidase enzymatic
activity is a serum non-specific factor acting during the early and late stages of DM.
Also this study aimed to compare the effect of raising blood sugar with levels of CRP
& CP in DM2 patients.
The Glucose kit method & CRP latex were used in present study, CRP kit
which is based on immunological reaction between CRP antisera bound to
biologically inert latex particles and CRP in the test specimens of patients with DM
(13Female, 17Male median age years, range=30-65)yr & in control subjects (7F,14M
,age range=30-45) yr.

The results are classified according to visible agglutination to:
1- A positive result / is indicated by the obvious agglutination pattern of the latex, in a
clear solution.
2- A negative result: is indicated by no change in the latex suspension on the test
slide.
Then the results of the precipitin test correlated with the quantitative data on
CRP.
Ceruloplasmin oxidase activity measured by using the modified Rice method
& phenylene diamine – 2HCl as a substrate .
This study has found that CRP concentrations in patients with DM increased
very clearly than normal subjects, and established that CRP conc. in male was more
than in female for patients specimens. An increase in serum ceruloplasmin (CP)
oxidase activity has also been indicated in both sex, higher activity noted in female
than male.
Abbreviations :CRP:C-reactive protein, mg/dl: milligram per diacelitter,
BS:blood suger, CP: ceruloplasm in, F: Female, M: Male.
DM2: Type Two Diabetes Mellitus