Spectrophotometric Determination of Methyl dopa by Reaction with Tyramine and Potassium metaperiodate.

Abstract

A batch and flow injection (FI) spectrophotometric methods have been
developed for the determination of methyl dopa in aqueous solution and in
pharmaceutical tablets preparation. The methods are based on the reaction of methyl
dopa with tyramine in the presence of potassium metaperiodate as oxidizing agent.
The water soluble orange colour produced was measured at λmax 481 nm.linearity
was observed from 0.5-20 and from 1-50 μg ml^-1 methyl dopa with quantification
limits of 0.20 and 0.43 μg- ml^-1 methyl dopa by batch and FI procedure respectively.
The effect of chemical and physical parameters have been carefully considered and
the proposed procedures were successfully applied to the determination of methyl
dopa in tablet pharmaceutical formulation.