Spectrophtometric determination of Sulfadiazine in Various Sample by coupling with 2,5-dimethoxy aniline

Abstract

A simple, accurate and sensitive spectrophotometric method is developed for the quantitative determination of sulfadiazine (SDZ) in both pure and dosage forms. The method is based on diazotization of primary amine group of sulfadiazine with sodium nitrite and hydrochloric acid followed by coupling with 2,5-dimethoxy aniline to form a orang colored azo dye which shows maximum absorption at (478) nm. Beer’s law is obeyed over the concentration range of (0.1-5) μg.ml-1 of sulfadiazine, with molar absorptivity of (8,26×104) L mol-1 cm-1, and Sandell sensitivity index of (0.003) μg/cm2. The method does not need to temperature control. The optimum conditions for all color development are described and proposed methods were successfully applied to the determination of sulfadiazine in its pharmaceutical preparations (burn cream).
Key words: Spectrophotometric determination, Diazotization and coupling, Sulfadiazine , 2,5- dimethoxy aniline.