Simultaneous Spectrophotometric Determination of Mesalasine and Isoniazid Using H-Point Standard Addition Method via Schiff’s Base Formation Reaction

Abstract

H-point standard addition method (HPSAM) has been applied to the simultaneous spectrophotometric determination of mesalasine (5-ASA) and isoniazid (INH) drugs in binary mixtures. The method is based on the Schiff's base formation of drugs with 1,2-naphoquinone sulphonate (NQS) as a chromogenic reagent in the presence of micellar cityltrimethyl ammonium bromide (CTAB) and sodium carbonate. The results showed that simultaneous determinations could be performed with the ratios 0.4:1.4 and 1.6:0.2 measured at pair of wavelengths (438 and 521 nm) with recovery % range 94.5-105 % and precision better than 2.95 %