Application of High Performance Liquid Chromatography to the Analysis of Hydrochlorothiazide in Bulk and Pharmaceutical Formulations

Abstract

Hydrochlorothiazide is a common blood pressure medicine. To analysis of it as pure form
and in pharmaceutical formulations we used isocratic HPLC techniques supplied with a
Octadecyl silane (ODS or C18) column (4.5mm x 250 mm, 5μm) as stationary phase and
(Methanol/Acetonitrile/Water) in the ratio of [75: 15:10 v/v/v/] as mobile phase at (25 Cº). The
Hydrochlorothiazide was detected at 270 nanometer and a flow rate was at 1.0 ml.min-1. This
method was validated (Linearity, Accuracy, Precision, LOD and LOQ) and all results that
obtained refer to accepted within ICH Guidelines.